Vijay Shankar has been ruled out of the rest of the tournament with a toe fracture, the BCCI announced on Monday.
"Vijay Shankar sustained a non-displaced fracture of the left big toe, which will require a minimum of three weeks to heal. The injury rules him out of the ongoing World Cup," said a BCCI statement.
"The Indian team management has requested the ICC (International Cricket Council) to consider Mayank Agarwal as his replacement."
Shankar was struck on the foot by a yorker from Jasprit Bumrah during a training session and was not picked in the playing XI for India's 31-run defeat to England at Edgbaston on Sunday.
India have been struck with injuries in the World Cup with star opening batsman Shikhar Dhawan ruled out of the tournament with a broken thumb.
Fast bowler Bhuvneshwar Kumar had to miss out as well after suffering a hamstring strain against Pakistan and has not played since.
But he will be available for selection for India's next encounter against Bangladesh on Tuesday.
India need to win one of their remaining two matches to clinch a spot in the top four.