Justin Langer confirmed that Usman Khawaja will miss the rest of the tournament with a hamstring strain.
Khawaja suffered hamstring injury in Australia's clash against South Africa at Manchester.
Matthew Wade is set to replace Khawaja in the 15-man squad, subject to confirmation from the ICC.
"It's such a pity for Khawaja, for someone who's been so integral to how we've been playing," Langer told Cricket Australia's website on Sunday.
"Like Shaun (Marsh, who broke his arm on Thursday) I feel that sad for him that he's going to miss the World Cup semi-final.
"Matthew Wade is coming up, he's in great form, he's had a great 12 or 18 months back home but he also just scored two hundreds (for Australia A) so he's in great nick."
Meanwhile, Marcus Stoinis’ injury problems came back against South Africa which have already forced him to miss out two games.
All-rounder Mitchell Marsh has been called up to the squad as cover for Stoinis.
"Mitch Marsh is going to come on standby for Marcus Stoinis just to see how he comes up. If they have to come in they'll be ready to step up," Langer added.