James Anderson has been ruled out of the remainder of Ashes with a calf injury, the ECB said in a statement on Friday.
Anderson hasn’t played in the Ashes since breaking down after bowling only four overs in the first Test at Edgbaston.
There had been speculations about the Lancashire fast bowler featuring in the upcoming fourth Test at Old Trafford.
But England’s hopes were quashed when he felt pain in his right calf whilst playing for Lancashire’s second XI against Durham in a friendly match.
"England and Lancashire seamer James Anderson has been ruled out for the rest of the Specsavers Ashes series," said ECB in a statement.
"Whilst bowling in the ninth over on Thursday in Lancashire's second XI four-day friendly against Durham at Chester Boughton Hall Cricket Club, Anderson became aware of right calf pain following on from 20 overs he had bowled on Tuesday.
"He immediately sought a medical assessment from Lancashire and was withdrawn from the remainder of the game," the statement added.
"Further review with the ECB medical team has confirmed that he will be unavailable for the remaining two Specsavers Ashes Tests."
England have added Somerset quick Craig Overton into the squad.
The series is currently placed at 1-1 following England’s stunning one-wicket win in the third Test at Headingley.
Joe Root (capt), Jofra Archer, Jonny Bairstow, Stuart Broad, Rory Burns, Jos Buttler, Sam Curran, Joe Denly, Jack Leach, Craig Overton, Jason Roy, Ben Stokes, Chris Woakes