Temba Bavuma has been ruled out of the T20I series opener against Australia after sustaining a hamstring strain.
Bavuma suffered the injury while fielding during the third T20I against England at Centurion. He was forced off the field and sent for scans although he was later named in the squad for the three-match T20I series against Australia.
Bavuma opened the batting for South Africa against England, forming a formidable combination at the top of the order alongside skipper Quinton de Kock.
He racked up a sum of 123 runs in three matches, scoring at a rapid rate of 153.75 as he reinforced his white-ball credentials for the Proteas.
The recommended recovery time for Bavuma is seven to 10 days as he will continue to receive treatment from the medical staff.
The 29-year-old will remain with the team in the hopes of making a full recovery by Sunday's T20 fixture in Port Elizabeth.
South Africa have thus announced no replacement for Bavuma with either Jon-Jon Smuts, Rassie van der Dussen or Heinrich Klaasen expected to be called on as the stand-in opener.
There is a possibility that former captain Faf du Plessis could be chosen in the opening slot given his past experience of facing the new ball in the Indian Premier League for Chennai Super Kings.
Du Plessis made a surprise return to South Africa's T20I squad after being overlooked for selection for the England T20Is where the hosts succumbed to a 2-1 series defeat in closely-contested encounters.
The Proteas are currently experimenting with different combinations ahead of the T20 World Cup in October this year with talk of AB de Villiers' potential international return intensifying in recent times.