Ben Stokes's Ashes hopes were in the balance Thursday after England suspended him from international matches "until further notice" after a video emerged apparently showing him fighting outside a nightclub.
The 26-year-old Durham all-rounder was released without charge on Monday but remains under investigation.
The Sun newspaper published the footage from outside a nightclub in the southwest city of Bristol late on Wednesday.
Opening batsman Alex Hales, who was with Stokes on Sunday after playing alongside him during a win over West Indies in the third one-day international in Bristol, has also been suspended from England international selection.
"Ben Stokes and Alex Hales will not be considered for selection for England international matches until further notice," said an ECB statement.
"Each remains on full pay pending further ECB investigation and the ongoing police investigation into an incident in Bristol in the early hours of Monday 25 September.
"Andrew Strauss, director of England cricket, will today refer the internal disciplinary procedure for these two players to the Cricket Discipline Commission, chaired by Tim O’Gorman."
The statement added: "These decisions, fully supported by ECB chairman Colin Graves, were made following the release of footage viewed by ECB for the first time on Wednesday night."
Neither Hales, who returned voluntarily to Bristol on Tuesday to give further evidence to police about the incident, nor Stokes were selected for Wednesday's six-run win over West Indies in the fourth one-day international at The Oval that gave England an unbeatable 3-0 series lead.
Stokes's hand injury may well have meant he was physically unfit for selection in any case.
Dawid Malan has been added as cover to the squad for the Southampton match after Hales was ruled out.
Ashes-holders England's next international fixture will be the first Test against Australia in Brisbane commencing on November 23.
Hales, who last played Test cricket over a year ago, was not included in the Ashes squad.
England coach Trevor Bayliss said Wednesday he was disappointed that Stokes had been out as late as 2:35 am (0135 GMT), when police were called to the incident, and said team management would look at whether they needed to impose a curfew during the five-Test Ashes series.
But the Australian defended retaining Stokes as vice-captain to Test skipper Joe Root, saying: "What he has shown for us is that he is a leader within this group when it comes to cricket.
"At the moment I am quite happy for him to be the vice-captain," Bayliss added.
Stokes, potentially just one on-field offence away from a Test ban under the International Cricket Council's code of conduct, is seen as vital to England's chances of retaining the Ashes, with former England captain Michael Vaughan, saying: "I don't think England can win in Australia without Ben Stokes."
This is not the first time Stokes has been involved in a controversial off-field incident.
In 2011 he was arrested and later cautioned for obstructing police on a pre-Christmas night out.
And in February 2013 he was sent home from an England A team tour of Australia for flouting rules on late-night drinking.
In June last year Stokes was fined after he was caught speeding for a fourth time and warned a further offence could lead to a prison sentence.
Stokes told The Times in an interview published on Saturday he enjoyed a couple of beers after stumps.
"We're grown men, go out for dinner, have a few pints," he said. "I'm 26, not 14. I don't have to drink Diet Cokes with dinner."