The injury to Roy prompted England to choose different opening pairs for the fixtures against Pakistan and Australia. Tom Banton and Jonny Bairstow were handed the duties at the top of the order for the Pakistan clashes whereas the experiment to open with Jos Buttler continued for the first two Australia T20Is.
Despite the injury setback, Roy had stayed with the England squad to undergo rehabilitation and has now recovered in time for the 50-over assignment against Aaron Finch's men. Roy recently pulled out of the Indian Premier League as well as Delhi Capitals called up Daniel Sams as his replacement.
The other development regarding England's ODI line-up is the inclusion of world No. 1 T20I batsman Dawid Malan as part of the reserves.
Malan replaces Joe Denly in the set-up, though the southpaw has played only one ODI. Denly has been released from England's bio-secure bubble to participate in the T20 Blast.
"Malan has come in as a reserve, Roy has come through fit so he will join the squad and obviously Salt has come in as a reserve as well," England coach Chris Silverwood said.
Buttler was not available for the third T20I due to personal reasons but is expected to feature in the ODIs pending the result of his Covid-19 test prior to reintegrating with the team.
Skipper Eoin Morgan had dislocated his finger during the second T20I at Southampton and did not take part in the series finale, which gave the chance to Moeen Ali to captain England for the first time.
Nevertheless, Morgan will be leading his side once the ODI series commences on Friday at Old Trafford.
"Jos will be back in the bubble to join us in Manchester, baring Test results. He should be available to play. Eoin, obviously good news around his finger, and touch wood he'll be fit as well."
Eoin Morgan (c), Moeen Ali, Jofra Archer, Jonny Bairstow (wk), Tom Banton, Sam Billings, Jos Buttler (wk), Sam Curran, Tom Curran, Adil Rashid, Joe Root, Jason Roy, Chris Woakes, Mark Wood
Reserves: Dawid Malan, Phil Salt, Saqib Mahmood